Antibodies for Rare Cell and Circulating Tumor Cells (CTC) Enumeration

s

s

s

The Genesis System with Celselect Slides™ can be used for efficient capture, recovery, and analysis of rare cells, circulating tumor cells (CTCs) from liquid biopsy samples, cell culture, and other liquid sample types.

The system uses gentle microfluidics and size-based cell selection, followed by antibody-based immunostaining.

This video outlines the system and the use of antibodies in cell enumeration.

Antibodies are the key reagent for identifying your target cell types, requiring careful selection to ensure that the antibody will not only detect the required cell marker but is also compatible with the Genesis System and the Celselect Slides.

Antibody Selection and Preparation for Circulating Tumor Cell (CTC) and Rare Cell Isolation Using the Celselect Slides Enumeration Assay

Read the Guide

Read the Antibody Selection and Preparation for Circulating Tumor Cell (CTC) and Rare Cell Isolation Using the Celselect Slides Enumeration Assay Quick Guide that takes you through key considerations for creating an optimized staining panel.

Validated Antibodies

Antibodies have been validated for use for rare cell and CTC enumeration (EN) on the Genesis Cell Isolation System with the Celselect Slide technology. Here, we show an example staining of a blood sample spiked with MCF-7 cells. After following the antibody selection and preparation guide, antibodies were used at the optimal dilution in the indirect staining workflow for Celselect Slides EN. Leukocytes were identified using Mouse Anti-Human CD45 Antibody (MCA87) and detected using a DyLight 550 conjugated Human Anti-Mouse IgG2a Secondary Antibody (HCA310D550).

Immunolabeling of MCF-7 cells was performed using Mouse Anti-Human Cytokeratin (Pan reactive) Antibody (MCA6434GA) and detected using a FITC conjugated Goat Anti-Mouse IgG1 Secondary Antibody (STAR132F). The resulting readout is displayed in Figure 1. PanCK staining was seen in the FITC (green) channel clearly showing presence of MCF-7 cells without the contaminating presence of leukocytes, as shown by the lack of CD45 staining in the DyLight (red) channel. DAPI was included to show the presence of all nuclei containing cells as a control, showing in the merged image that the MCF-7 cells match exactly with the nucleated cells.

Fig.1. Immunofluorescent Staining of MCF-7 Cells Spiked Blood Samples on the Genesis System Using Celselect Slides. MCF-7 cells (green) stained with anti PanCK antibodies were detected using a FITC conjugated secondary and seen in the FITC (GFP) channel. Leukocytes (red) stained with anti CD45 antibodies were detected using a DyLight 550 conjugated secondary and seen in the DyLight 550 (TRITC) channel. Imaged using Nikon Ti2 at 20x magnification.

Primary Antibodies

Secondary Antibodies

All validated antibodies have an EN application stated in the antibodies guaranteed application and show the EN logo on the product page: