Alexa Fluor ® Conjugates

lexa Fluor® fluorescent conjugates give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy. Alexa Fluor® conjugates are:

  • Brighter and more fluorescent than comparable spectral conjugates
  • More photostable than other fluorochromes
  • Insensitive to changes in pH over a broad range
  • Ideal for multicolor applications due to minimal spectral overlap
  • Compatible with all common fluorescence detection channels

Key Alexa Fluor® Conjugates

Four of the most useful Alexa Fluor® conjugates: 4 05, 48 8, 64 7 and 70 0 are widely available across our human, mouse, and rat product catalog ranges.

Choose from the list below to view the current primary antibodies available conjugated to Alexa Fluor® dyes.

Human: Alexa Fluor ® 405 Alexa Fluor ® 488 Alexa Fluor ® 647 Alexa Fluor ® 700
Mouse: Alexa Fluor ® 405 Alexa Fluor ® 488 Alexa Fluor ® 647 Alexa Fluor ® 700
Rat: Alexa Fluor ® 405 Alexa Fluor ® 488 Alexa Fluor ® 647 Alexa Fluor ® 700

Technical Features of Alexa Fluor® Conjugates

Fluorochrome Maximal absorbance Maximal emission Flow cytometry Confocal microscopy Color Common detection channels
Alexa Fluor ® 405 401 421 Yes Yes Blue FL-4, FL-6, FL-9
Alexa Fluor ® 488 495 519 Yes Yes Green FL-1
Alexa Fluor ® 647 650 665 Yes Yes Red FL-4, FL-5, FL-6, FL-8
Alexa Fluor ® 700 702 723 Yes Yes InfraRed  

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Alexa Fluor® 405

  • The best blue fluorochrome available
  • Potentially more intense in fluorescence than other UV light-excitable blue conjugates
  • Excited by Blue Diode, Violet, or Krypton lasers
  • Usually detected in the same fluorescence channel as Cascade Blue
  • Minimal overlap with green fluorophores

Alexa Fluor® 488

  • The finest green fluorophore available
  • Excited by an Argon-Ion laser
  • Usually detected in the same fluorescence channel as FITC
  • Alternative for fluorescein conjugates (FITC, FAM) and Cy2
  • Brighter and more photostable than fluorescein

alexa3Alexa Fluor® 647

  • A very high quality red/purple dye
  • Replaces APC (allophycocyanin) or Cy5
  • Excited by Helium-Neon or Krypton-Neon lasers
  • Usually detected in the same fluorescence channel as APC
  • Total fluorescence significantly higher than Cy5 dyes
  • More photostable than DPXL-3, Cy5, and APC conjugates

Alexa Fluor® 700

  • A near infrared dye
  • Excited by xenon-arc lamp, far red diode lasers or dye-pumped lasers (675-700 nm)
  • Usually detected in the same fluorescence channel as APC
  • Brighter than Cy5, Cy5.5, Cy7, and similar dyes
  • More photostable than Cy5 and APC conjugates

All AbD Serotec products are backed by our Performance Guarantee and supported by unrivalled pre- and post-sales technical support, so you can trust AbD Serotec for all your Alexa Fluor® conjugated antibodies.


Alexa Fluor ® is a registered trademark of Molecular Probes Inc. OR, USA

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