DyLight® Dyes

DyLight® Dyes

Newborn rat primary cardiac myocytes stained with mouse anti-desmin, detected with goat anti-mouse IgG:DyLight® 488 (STAR74D488); rat anti-alpha tubulin detected with goat anti-rat IgG:DyLight® 649 (STAR71D649); and rabbit anti-MyBP-C detected with sheep anti-rabbit IgG: DyLight® 549 (STAR36D549).

DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.

  • More intense fluorescence and sensitivity than comparable spectral conjugates
  • Economical as DyLight® dyes require less conjugated antibody to study your cells than FITC and RPE
  • Greater photostability than other fluorochromes
  • Good stability over pH 4 - 9
  • Ideal for immunofluorescence techniques
  • Compatible with all common fluorescence detection channels

DyLight® dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry, and may also be used in ELISA, Western blotting, high throughput screening and other array platforms.

Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.

We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.

 

Dye Excitationmax (nm) Emissionmax (nm)  Fluorescence color
DyLight® 405 400 405 Blue
DyLight® 488 493 518 Green
DyLight® 550 562 576 Yellow
DyLight® 650 652 672 Red
DyLight® 680 692 712 Near IR
DyLight® 800 777 794 Near IR

DyLight® Dyes

  • Format: DyLight®405|DyLight®488|DyLight®550|DyLight®650|DyLight®680|DyLight®800|DyLight®549|DyLight®649
DescriptionSpecificityTarget SpeciesFormatHostIsotypeCloneApplicationsProduct TypeProduct Code
Human anti Human Actin beta:DyLight®680ACTIN BETAHumanDyLight®680HuCALHuCAL Fab bivalent12141F, WBMonoclonal AntibodyHCA147D680
Human anti Human Actin beta:DyLight®800ACTIN BETAHumanDyLight®800HuCALHuCAL Fab bivalent12141WBMonoclonal AntibodyHCA147D800
Mouse anti c-Myc:DyLight®549C-MYCHumanDyLight®549MouseIgG19E10F, IFMonoclonal AntibodyMCA2200D549GA
Human anti Cyclophilin A:DyLight®680CYCLOPHILIN AHumanDyLight®680HuCALHuCAL Fab bivalent794WBMonoclonal AntibodyHCA005D680
Human anti Cyclophilin A:DyLight®800CYCLOPHILIN AHumanDyLight®800HuCALHuCAL Fab bivalent794WBMonoclonal AntibodyHCA005D800
Mouse anti Rabbit GAPDH:DyLight®680GAPDHRabbitDyLight®680MouseIgG16C5WBMonoclonal AntibodyMCA4739D680
Mouse anti Rabbit GAPDH:DyLight®800GAPDHRabbitDyLight®800MouseIgG16C5WBMonoclonal AntibodyMCA4739D800
Mouse anti Histidine Tag:DyLight®549HISTIDINE TAGSynthetic PeptideDyLight®549MouseIgG1AD1.1.10F, IFMonoclonal AntibodyMCA1396D549GA
Mouse anti Histidine Tag:DyLight®800HISTIDINE TAGSynthetic PeptideDyLight®800MouseIgG1AD1.1.10WBMonoclonal AntibodyMCA1396D800GA
Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)IgG MouseDyLight®405GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR117D405GA
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)IgG MouseDyLight®488GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR117D488GA
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)IgG MouseDyLight®549GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR117D549GA
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)IgG MouseDyLight®649GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR117D649GA
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)IgG MouseDyLight®800GoatPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR117D800GA
Rabbit F(ab')2 anti Rat IgG:Dylight®405IgGRatDyLight®405RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR16D405GA
Donkey anti Sheep/Goat IgG:Dylight®405IgGSheepDyLight®405DonkeyPolyclonal IgGF, IFPolyclonal AntibodySTAR88D405GA
Goat anti Mouse IgG:Dylight®405 (Rat Adsorbed)IgGMouseDyLight®405GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR74D405GA
Goat F(ab')2 anti Hamster IgG:Dylight®405IgGHamsterDyLight®405GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR104D405GA
Rabbit F(ab')2 anti Mouse IgG:Dylight®405IgGMouseDyLight®405RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR8D405GA
Goat F(ab')2 anti Hamster IgG:DyLight®488IgGHamsterDyLight®488GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR104D488GA
Donkey anti Sheep/Goat IgG:DyLight®488IgGSheepDyLight®488DonkeyPolyclonal IgGF, IFPolyclonal AntibodySTAR88D488GA
Rabbit F(ab')2 anti Rat IgG:DyLight®488IgGRatDyLight®488RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR16D488GA
Rabbit F(ab')2 anti Mouse IgG:DyLight®488IgGMouseDyLight®488RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR8D488GA
Goat anti Mouse IgG:DyLight®488 (Rat Adsorbed)IgGMouseDyLight®488GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR74D488GA
Sheep anti Rabbit IgG:DyLight®488IgGRabbitDyLight®488SheepPolyclonal IgGF, IFPolyclonal AntibodySTAR36D488GA
Rabbit F(ab')2 anti Rat IgG:DyLight®549IgGRatDyLight®549RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR16D549GA
Rabbit F(ab')2 anti Mouse IgG:DyLight®549IgGMouseDyLight®549RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR8D549GA
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)IgGRatDyLight®549GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR71D549GA
Donkey anti Sheep/Goat IgG:DyLight®549IgGSheepDyLight®549DonkeyPolyclonal IgGF, IFPolyclonal AntibodySTAR88D549GA
Goat anti Mouse IgG:DyLight®549 (Rat Adsorbed)IgGMouseDyLight®549GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR74D549GA
Goat F(ab')2 anti Hamster IgG:DyLight®549IgGHamsterDyLight®549GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR104D549GA
Sheep anti Rabbit IgG:DyLight®549IgGRabbitDyLight®549SheepPolyclonal IgGF, IFPolyclonal AntibodySTAR36D549GA
Goat F(ab')2 anti Hamster IgG:DyLight®649IgGHamsterDyLight®649GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR104D649GA
Goat anti Mouse IgG:DyLight®649 (Rat Adsorbed)IgGMouseDyLight®649GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR74D649GA
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)IgGRatDyLight®649GoatPolyclonal IgGF, IFPolyclonal AntibodySTAR71D649GA
Rabbit F(ab')2 anti Mouse IgG:DyLight®649IgGMouseDyLight®649RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR8D649GA
Donkey anti Sheep/Goat IgG:DyLight®649IgGSheepDyLight®649DonkeyPolyclonal IgGF, IFPolyclonal AntibodySTAR88D649GA
Rabbit F(ab')2 anti Rat IgG:DyLight®649IgGRatDyLight®649RabbitPolyclonal IgGF, IFPolyclonal AntibodySTAR16D649GA
Rabbit F(ab')2 anti Mouse IgG:Dylight®800IgGMouseDyLight®800RabbitPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR8D800GA
Goat F(ab')2 anti Hamster IgG:Dylight®800IgGHamsterDyLight®800GoatPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR104D800GA
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)IgGRatDyLight®800GoatPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR71D800GA
Goat anti Mouse IgG:Dylight®800 (Rat Adsorbed)IgGMouseDyLight®800GoatPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR74D800GA
Donkey anti Sheep/Goat IgG:Dylight®800IgGSheepDyLight®800DonkeyPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR88D800GA
Rabbit F(ab')2 anti Rat IgG:Dylight®800IgGRatDyLight®800RabbitPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR16D800GA
Sheep anti Rabbit IgG:Dylight®800IgGRabbitDyLight®800SheepPolyclonal IgGF, IF, WBPolyclonal AntibodySTAR36D800GA
Human anti Maltose Binding Protein:DyLight® 549MALTOSE BINDING PROTEINBacterialDyLight®549HuCALHuCAL Fab bivalent6592E, IFMonoclonal AntibodyHCA089D549
Rat IgG2a Negative Control:DyLight® 649RAT IgG2a NEGATIVE CONTROLNegative ControlDyLight®649RatIgG2aFNegative/Isotype ControlMCA1212D649
Streptavidin:DyLight®405STREPTAVIDINDyLight®405FAccessory ReagentSTAR148D405GA
Streptavidin:DyLight®488STREPTAVIDINDyLight®488FAccessory ReagentSTAR149D488GA
Streptavidin:DyLight®549STREPTAVIDINDyLight®549FAccessory ReagentSTAR150D549GA
Streptavidin:DyLight®649STREPTAVIDINDyLight®649FAccessory ReagentSTAR151D649GA
Streptavidin:DyLight®800STREPTAVIDINDyLight®800F, WBAccessory ReagentSTAR152D800GA
Rat anti Tubulin alpha:DyLight®680TUBULIN ALPHAYeastDyLight®680RatIgG2aYL1/2WBMonoclonal AntibodyMCA77D680
Rat anti Tubulin alpha:DyLight®800TUBULIN ALPHAYeastDyLight®800RatIgG2aYL1/2WBMonoclonal AntibodyMCA77D800
Mouse anti V5-Tag:DyLight®405V5-TAGViralDyLight®405MouseIgG2aSV5-Pk1F, IFMonoclonal AntibodyMCA1360D405GA
Mouse anti V5-Tag:DyLight®549V5-TAGViralDyLight®549MouseIgG1SV5-Pk4F, IFMonoclonal AntibodyMCA2894D549GA
Mouse anti V5-Tag:DyLight®549V5-TAGViralDyLight®549MouseIgG1SV5-Pk5F, IFMonoclonal AntibodyMCA2895D549GA
Mouse anti V5-Tag:DyLight®550V5-TAGViralDyLight®550MouseIgG2aSV5-Pk1IFMonoclonal AntibodyMCA1360D550GA
Mouse anti V5-Tag:DyLight®649V5-TAGViralDyLight®649MouseIgG1SV5-Pk5F, IFMonoclonal AntibodyMCA2895D649GA
Mouse anti V5-Tag:DyLight®649V5-TAGViralDyLight®649MouseIgG1SV5-Pk4F, IFMonoclonal AntibodyMCA2894D649GA

Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.