Alexa Fluor ® Conjugates
Alexa Fluor® fluorescent conjugates give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy. Alexa Fluor® conjugates are:
- Brighter and more fluorescent than comparable spectral conjugates
- More photostable than other fluorochromes
- Insensitive to changes in pH over a broad range
- Ideal for multicolor applications due to minimal spectral overlap
- Compatible with all common fluorescence detection channels
Key Alexa Fluor® Conjugates
Four of the most useful Alexa Fluor® conjugates: 405, 488, 647 and 700 are widely available across our human, mouse, and rat product catalog ranges. Choose from the list below to view the current primary antibodies available conjugated to Alexa Fluor® dyes.
Human: | ||||
Mouse: | ||||
Rat: | Alexa Fluor ® 405 |
We also offer custom conjugation of these Alexa Fluors®, including the tandem dyes PE-Alexa Fluor® 647, PE-Alexa Fluor® 750 and APC-Alexa Fluor® 750 to any of our primary antibody catalog products. Not all antibody suppliers can offer you this Custom Service; and this is just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.
Technical Features of Alexa Fluor® Conjugates
Fluorochrome | Maximal absorbance | Maximal emission | Flow cytometry | Confocal microscopy | Color | Common detection channels |
|---|---|---|---|---|---|---|
Alexa Fluor ® 405 | 401 | 421 | Yes | Yes | Blue | FL-4, FL-6, FL-9 |
Alexa Fluor ® 488 | 495 | 519 | Yes | Yes | Green | FL-1 |
Alexa Fluor ® 647 | 650 | 665 | Yes | Yes | Red | FL-4, FL-5, FL-6, FL-8 |
Alexa Fluor ® 700 | 702 | 723 | Yes | Yes | InfraRed |
Alexa Fluor® 405
- The best blue fluorochrome available
- Potentially more intense in fluorescence than other UV light-excitable blue conjugates
- Excited by Blue Diode, Violet, or Krypton lasers
- Usually detected in the same fluorescence channel as Cascade Blue
- Minimal overlap with green fluorophores
Alexa Fluor® 488
- The finest green fluorophore available
- Excited by an Argon-Ion laser
- Usually detected in the same fluorescence channel as FITC
- Alternative for fluorescein conjugates (FITC, FAM) and Cy2
- Brighter and more photostable than fluorescein
Alexa Fluor® 647
- A very high quality red/purple dye
- Replaces APC (allophycocyanin) or Cy5
- Excited by Helium-Neon or Krypton-Neon lasers
- Usually detected in the same fluorescence channel as APC
- Total fluorescence significantly higher than Cy5 dyes
- More photostable than DPXL-3, Cy5, and APC conjugates
Alexa Fluor® 700
- A near infrared dye
- Excited by xenon-arc lamp, far red diode lasers or dye-pumped lasers (675-700 nm)
- Usually detected in the same fluorescence channel as APC
- Brighter than Cy5, Cy5.5, Cy7, and similar dyes
- More photostable than Cy5 and APC conjugates
All AbD Serotec products are backed by our Performance Guarantee and supported by unrivalled pre- and post-sales technical support, so you can trust AbD Serotec for all your Alexa Fluor® conjugated antibodies.
Alexa Fluor ® is a registered trademark of Molecular Probes Inc. OR, USA
