Human Flow Cytometry Products and Resources

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To aid in your experiments we have collated our human-specific products, including Seroblock and our range of human antibodies, alongside specific resources, such as our Human Cell Marker Selection Tool.

Fig. 1. Staining of THP-1 cells with FITC labelled Mouse anti Human CD11a (MCA1848) or Mouse IgG2a Negative Control:FITC (MCA929F) in the absence (A) and presence (B) of Human SeroBlock (BUF070A).

Fig. 1. Staining of THP-1 cells with FITC labelled Mouse anti-Human CD11a (MCA1848) or Mouse IgG2a Negative Control:FITC (MCA929F) in the absence (A) and presence (B) of Human SeroBlock (BUF070A).

Products

Human Seroblock

Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes, and dendritic cells.

Cells that express Fc receptors can give false positive immunofluorescent staining due to Fc receptor Ig binding.

Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.

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Human Flow Cytometry Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Resources

    CD Marker Tool

    Interactive Human Immune Cell Marker Database

    Our interactive online tool is easy to use and can help you

    • Look up immune cell-specific markers
    • Choose a cell lineage
    • Examine detailed data for 440 markers
    • Discover immune cell marker expression
    • Find the antibody to your marker
       

    Try Human Tool

    Fig. 2. Staining human mononuclear cells. Viable cells were identified by PureBlu DAPI (1351303) staining. Human peripheral blood was stained with CD63 FITC (MCA2142F) and CD14 SBV670 (MCA1568SBV670) and gated on mononuclear cells. Data acquired on the ZE5 Cell Analyzer.

    Fig. 2. Staining human mononuclear cells. Viable cells were identified by PureBlu DAPI (1351303) staining. Human peripheral blood was stained with CD63 FITC (MCA2142F) and CD14 SBV670 (MCA1568SBV670) and gated on mononuclear cells. Data acquired on the ZE5 Cell Analyzer.

    Cell Frequencies in Common Samples

    Cell frequency can influence the choice of fluorophore and how many cells you will need to collect to obtain statistically significant data, crucial with rare populations.

    We have produced a handy table showing the relative frequency of cells in commonly used human tissues, allowing you to plan and predict your flow populations.

    Read our Full Guide

    Human Cell Frequency — Peripheral Blood

    Common Leukocytes Lymphoid Cells Myeloid Cells

    Cell Type

    Percent

    Cell Type

    Percent

    Cell Type

    Percent

    T cells

    10-25

    CD4+

    10-20

    Neutrophils

    40-65

    B cells

    3-10

    CD8+

    3-10

    Eosinophils

    0.1-2

    Granulocytes

    45-65

    Tregs

    0.2-1.4

    Basophils

    0.1-2

    Monocytes

    3-10

    γδ T cells

    1-5

    Dendritic cells (DC)

    0.5-1

    NK cells

    2-5

    Naïve B cells

    0.5-5

    Monocyte DCs

    0.2-0.4

    Dendritic cells (DC)

    0.5-1

    Memory B cells

    0.4-2

    Plasmacytoid DCs

    0.1-0.2

    Stem cells

    0.01-0.05

    NKT cells

    0.01-0.5

    Innate lymphoid cells

    0.01-0.1

    These values are for reference only and there may be variation due to sample preparation and individual variation.

    All values shown are percentages of total leukocytes in each sample. 

    For more information about the importance of cell frequency in your flow cytometry experiment, please read our full guide.